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Evidence Guide: MSL974011 - Prepare tissue and cell cultures

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

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MSL974011 - Prepare tissue and cell cultures

What evidence can you provide to prove your understanding of each of the following citeria?

Work safely according to the legal and regulatory framework

  1. Ensure work practices and personal actions conform to regulations, codes, guidelines and workplace quality assurance procedures
  2. Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment
  3. Select, fit and use personal protective equipment (PPE)
  4. Address hazards and incidents as they arise
  5. Maintain a chain of custody, traceable to the worker, for all cells and tissues
Ensure work practices and personal actions conform to regulations, codes, guidelines and workplace quality assurance procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select, fit and use personal protective equipment (PPE)

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Address hazards and incidents as they arise

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a chain of custody, traceable to the worker, for all cells and tissues

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare and test cell and tissue culture media

  1. Select and confirm media specifications and processes/methods
  2. Prepare culture media to suit the application
  3. Sterilise culture media and check for sterility
  4. Perform quality control checks to ensure that culture media is fit for purpose
  5. Store culture media in accordance with specifications
Select and confirm media specifications and processes/methods

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare culture media to suit the application

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Sterilise culture media and check for sterility

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform quality control checks to ensure that culture media is fit for purpose

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Store culture media in accordance with specifications

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare tissue or cell cultures

  1. Select tissue/cell sample to optimise growth and prepare it for culture
  2. Add specified growth agents and/or nutrients
  3. Inoculate culture medium using aseptic techniques
Select tissue/cell sample to optimise growth and prepare it for culture

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Add specified growth agents and/or nutrients

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Inoculate culture medium using aseptic techniques

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Monitor tissue or cell culture

  1. Incubate culture in specified conditions
  2. Monitor growth of culture and record appearance and characteristics
  3. Report presence or absence of contamination
  4. Subculture the culture to continue the cell line
  5. Dispose of biohazardous and other laboratory waste safely
Incubate culture in specified conditions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Monitor growth of culture and record appearance and characteristics

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Report presence or absence of contamination

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Subculture the culture to continue the cell line

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Dispose of biohazardous and other laboratory waste safely

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain records

  1. Maintain records of batches of media and test data
  2. Ensure records of tissue cultures are retrievable, legible and accurate
  3. Ensure records conform to information management, records, quality system and legal requirements
Maintain records of batches of media and test data

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure records of tissue cultures are retrievable, legible and accurate

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure records conform to information management, records, quality system and legal requirements

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Evidence Guide

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Work safely according to the legal and regulatory framework

1.1

Ensure work practices and personal actions conform to regulations, codes, guidelines and workplace quality assurance procedures

1.2

Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment

1.3

Select, fit and use personal protective equipment (PPE)

1.4

Address hazards and incidents as they arise

1.5

Maintain a chain of custody, traceable to the worker, for all cells and tissues

2

Prepare and test cell and tissue culture media

2.1

Select and confirm media specifications and processes/methods

2.2

Prepare culture media to suit the application

2.3

Sterilise culture media and check for sterility

2.4

Perform quality control checks to ensure that culture media is fit for purpose

2.5

Store culture media in accordance with specifications

3

Prepare tissue or cell cultures

3.1

Select tissue/cell sample to optimise growth and prepare it for culture

3.2

Add specified growth agents and/or nutrients

3.3

Inoculate culture medium using aseptic techniques

4

Monitor tissue or cell culture

4.1

Incubate culture in specified conditions

4.2

Monitor growth of culture and record appearance and characteristics

4.3

Report presence or absence of contamination

4.4

Subculture the culture to continue the cell line

4.5

Dispose of biohazardous and other laboratory waste safely

5

Maintain records

5.1

Maintain records of batches of media and test data

5.2

Ensure records of tissue cultures are retrievable, legible and accurate

5.3

Ensure records conform to information management, records, quality system and legal requirements

Required Skills and Knowledge

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Work safely according to the legal and regulatory framework

1.1

Ensure work practices and personal actions conform to regulations, codes, guidelines and workplace quality assurance procedures

1.2

Identify hazards and workplace controls associated with the sample, preparation methods, reagents and equipment

1.3

Select, fit and use personal protective equipment (PPE)

1.4

Address hazards and incidents as they arise

1.5

Maintain a chain of custody, traceable to the worker, for all cells and tissues

2

Prepare and test cell and tissue culture media

2.1

Select and confirm media specifications and processes/methods

2.2

Prepare culture media to suit the application

2.3

Sterilise culture media and check for sterility

2.4

Perform quality control checks to ensure that culture media is fit for purpose

2.5

Store culture media in accordance with specifications

3

Prepare tissue or cell cultures

3.1

Select tissue/cell sample to optimise growth and prepare it for culture

3.2

Add specified growth agents and/or nutrients

3.3

Inoculate culture medium using aseptic techniques

4

Monitor tissue or cell culture

4.1

Incubate culture in specified conditions

4.2

Monitor growth of culture and record appearance and characteristics

4.3

Report presence or absence of contamination

4.4

Subculture the culture to continue the cell line

4.5

Dispose of biohazardous and other laboratory waste safely

5

Maintain records

5.1

Maintain records of batches of media and test data

5.2

Ensure records of tissue cultures are retrievable, legible and accurate

5.3

Ensure records conform to information management, records, quality system and legal requirements

Evidence of competence in this unit must satisfy all of the requirements of the elements and performance criteria, and include demonstration of:

safely preparing at least three (3) different primary tissue cultures that are free of contamination using tissue culture and basic subculture procedures

manipulating equipment and materials and samples to prevent contamination at all preparation stages

preparing, diluting and sterilising reagents and culture media that are fit for purpose

performing routine maintenance and checks on raw materials and consumables, including use-by-date, possible contamination and storage conditions

growing cell lines and tissue to specifications without contaminating the original sample and the environment

monitoring cell growth, checking growth rates and recognising problems, such as contamination of media by bacteria (e.g. Mycoplasma), fungi and other plant or animal tissue

identifying expected cell types and recognising normal and abnormal cells using an inverted microscope

accurately counting total and viable cells

maintaining chain of custody and traceability to the worker, of all cell lines, tissues, logs of work completed and procedures/methods used

working safely and satisfying all legal and regulatory requirements, including the use and care of biohazard cabinets.

Must provide evidence that demonstrates knowledge of:

basic classes and classification of culturable material, such as organisms, plants, animals, bacteria, viruses, tissues, cells and prions

structure and function of cells and organelles

cell structure, physiology and processes, including simple and facilitated diffusion, plasmolysis, osmosis, tonicity, active transport, energy production, mitosis, motility, phagocytosis and pinocystosis

concepts and principles of cell growth, including the need for nutrients, the role of growth regulators and the removal of wastes

types and sources of contamination

importance of strict aseptic techniques and cleaning procedures

quality control checks and quality assurance procedures

workplace and/or legal traceability requirements

hazards and risks in biological laboratories and relevant work health and safety (WHS) and environment requirements.

Range Statement

This field allows for different work environments and conditions that may affect performance. Essential operating conditions that may be present (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) are included.

Standards, codes, procedures and/or workplace requirements

Standards, codes, procedures and/or workplace requirements include the latest version of one or more of:

Australian and international standards covering the requirements for the competence of testing and calibration laboratories, laboratory safety, quality management and environmental management

Australian standards covering the requirements for cleaning, disinfecting and sterilising reusable medical and surgical instruments and equipment, and maintenance of associated environments in health care facilities

national work health and safety (WHS) standards and codes of practice, national environmental protection measures, and national measurement regulations and guidelines

specific codes, guidelines and procedures, such as National Association of Testing Authorities (NATA) accreditation requirements, principles of good laboratory practice (GLP), Australia New Zealand Food Standards (ANZFS) Code, Australian code of good manufacturing practice for medicinal products (GMP), Australian Dangerous Goods Code, gene technology regulations, and Guide to physical containment levels and facility types

workplace documents, such as standard operating procedures (SOPs); quality and equipment manuals; calibration and maintenance schedules; material safety data sheets (MSDS) and safety procedures; material, client and product specifications; production and laboratory schedules; workplace recording and reporting procedures; and waste minimisation and safe disposal procedures

workplace procedures and standard methods for preparing culture media, and operation and maintenance manuals for automated media preparation equipment

Applications of plant tissue/cell culture

Applications of plant tissue/cell culture include, but are not limited to, one or more of:

mass propagation of commercial species

production of disease-free plants by meristem tip culture

conservation of rare plants

haploid plant production by anther/pollen culture

'sports' produced by somaclonal variation

development of resistant plants by directed cell selection

protoplast fusion to produce novel plant hybrids

Applications of animal tissue/cell culture

Applications of animal tissue/cell culture include, but are not limited to, one or more of:

establishment and maintenance of animal cell lines, such as liver, epidermal and fibroblastic

maintenance of continuous cell lines

preparation of cell cultures for commercial sale

growth and enumeration of viruses

extraction of DNA

extraction of antigens for use in diagnostic tests

research of cell structure and function, cancer and tumour biology

immunofluorescent techniques

testing of media efficacy

production of monoclonal antibodies

production of genetically modified cell cultures

secondary metabolite production

Sterilisation and disposal of biohazardous wastes

Sterilisation and disposal of biohazardous wastes includes, but is not limited to, one or more of:

steam and high pressure air or steam

boiling, microwaving and autoclaving

filtration

gas, chemical and radiation

Plant tissues and cells

Plant tissues and cells include, but are not limited to, one or more of:

plant tissue, such as petioles, leaves, stems and petals

meristem tissue

special tissue, such as fern stolon, seed embryos and somatic embryoids

tissue for callus development to initiate cell suspension cultures

Animal tissues and cells

Animal tissues and cells include, but are not limited to, one or more of:

primary cells from animal tissue, such as heart, liver, kidney and epidermal

secondary cells, such as epithelial, endothelial and fibroblast

continuous cell lines, such as tumour lines, hybidomers and transformed lines (Epstein-Barr virus)

Preparing a primary culture

Preparing a primary culture includes, but is not limited to, one or more of:

thawing of cryopreserved cells and monitoring of cell recovery

enzymatic disaggregation from tissue

mechanical disaggregation from tissue

primary explant technique

pre-treatment

disinfestation of explants using hypochlorite and water

Suitable culture conditions

Suitable culture conditions include, but are not limited to, one or more of:

specified temperature and light intensity

appropriate atmosphere, such as carbon dioxide

shaking of cell suspensions or roller bottles

conditions for establishment, multiplication or planting out

special conditions for protoplast culture

Subculture

Subculture includes, but is not limited to, one or more of:

treatment of callus to multiply or regenerate shoots

treatment to encourage adventitious bud

treatment to encourage rooting

subculture of embryoids

cell suspensions

preparation of protoplasts

Safety procedures

Safety procedures include, but are not limited to, one or more of:

ensuring access to service shut-off points

recognising and observing hazard warnings and safety signs

labelling of samples, reagents, aliquoted samples and hazardous materials

handling and storage of hazardous materials and equipment in accordance with labelling, MSDS and manufacturer instructions

identifying and reporting operating problems or equipment malfunctions

cleaning and decontaminating equipment and work areas regularly

using PPE, such as gloves, safety glasses, coveralls and gowns

using containment facilities (PCII, PCIII and PCIV physical containment laboratories), containment equipment (biohazard containers, laminar flow cabinets, Class I, II and III biohazard cabinets) and containment procedures

following established manual handling procedures

reporting abnormal emissions, discharges and airborne contaminants, such as noise, light, solids, liquids, water/waste water, gases, smoke, vapour, fumes, odour and particulates, to appropriate personnel

WHS and environmental management requirements

WHS and environmental management requirements include:

· complying with WHS and environmental management requirements at all times, which may be imposed through state/territory or federal legislation. These requirements must not be compromised at any time

· applying standard precautions relating to the potentially hazardous nature of samples

· accessing and applying current industry understanding of infection control issued by the National Health and Medical Research Council (NHMRC) and State and Territory Departments of Health, where relevant